Coding

Part:BBa_K3890004:Design

Designed by: Beatriz Toledo Akiti   Group: iGEM21_USP-Brazil   (2021-09-30)


CYP6G1 fusioned with NADPH reductase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1947
    Illegal BglII site found at 3291
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 711
    Illegal BsaI.rc site found at 341
    Illegal BsaI.rc site found at 3242
    Illegal SapI.rc site found at 1822


Design Notes

sequences were added to the CYP6G1


Source

NCBI Drosophila melanogaster genome

References